High Perfomance Liquid Chromatography 

Technical Specifications

  • Optical Design: Tunable Czerny-Turner monochromator with internal reference beam
  • Light Source: Deuterium lamp, Tungsten lamp (optional)
  • Spectral Bandwidth: 6 nm at 254 nm
  • Wavelength Accuracy: ±1 nm
  • Wavelength Repeatability: ±0.1 nm
  • Wavelength Calibration: Internal calibration with the D-alpha line of the deuterium lamp
  • Wavelength Validation: Internal validation with holmium-oxide glass filter
  • Number of Signal Channels: Up to 2
  • Data Collection Rate:
    • Single-channel: Up to 125 Hz
    • Multi-channel: Up to 5 Hz
  • Noise:
    • Single-channel: <±2.5 µAU at 254 nm
    • Multi-channel: <±10 µAU (typical <±7 µAU) at 254 nm and 280 nm
  • Drift: <0.1 mAU/h at 254 nm
  • Linearity: <5% at 2.5 AU (typical <3% at 2.5 AU)

Flow Cell & Detector

  • Flow Cell Pressure Limits:
    • Standard & Semi-micro Biocompatible Flow Cell: 5 MPa (50 bar, 720 psi)
    • Semi-preparative Biocompatible Flow Cell: 10 MPa (100 bar, 1450 psi)
    • Standard & Semi-micro Flow Cell: 12 MPa (120 bar, 1740 psi)
  • Flow Range: 0.001-10 mL/min
  • Pressure Range: 2-70 MPa
  • Detector: UV-Vis
  • Wavelength Range: 190-750 nm
  • Wetted Parts: Fused Silica, PEEK, PTFE, SST

Safety & Connectivity

  • Safety Features:
    • Power-up diagnostics for optics, cooling fans, motors, and electronics
    • Leak detection with safe leak handling
  • PC Connection: USB 2.0 with a 3-port HUB for additional modules
  • I/O Interfaces: 2 × 6-pin Mini-DIN connectors (1 input, 1 relay out)
  • GLP Functions:
    • Predictive maintenance scheduling based on detector usage
    • Monitoring lamp age, ignitions, and intensity degradation
    • Leak detection & service monitoring
    • Full system parameter logging in Chromeleon CDS Audit Trail

Loading