Multi-Mode Microplate Reader

General Specifications

  • Detection Modes:
    • UV-Vis Absorbance
    • Fluorescence Intensity
    • Luminescence
    • Time-Resolved Fluorescence (Secondary Mode)
    • Alpha Detection
  • Microplate Types: Supports 6- to 384-well plates
  • Read Methods: End point, kinetic, spectral scanning, and well area scanning
  • Other Labware Supported: PCR plates, Petri and cell culture dishes, Take3 Micro-Volume Plates
  • Temperature Control:
    • 4-Zone™ Incubation up to 50°C with Condensation Control™
    • Accuracy: ±0.2°C at 37°C
  • Shaking Options: Linear, orbital
  • Software: Gen5™ Microplate Reader and Imager Software
  • Automation Compatibility: BioStack and 3rd party automation compatible

Absorbance Mode

  • Light Source: Xenon flash
  • Detector: Photodiode
  • Wavelength Selection: Monochromator
  • Wavelength Range: 200 – 999 nm (1 nm increments)
  • Monochromator Bandwidth: 2.4 nm
  • Dynamic Range: 0 – 4.0 OD
  • Resolution: 0.0001 OD
  • Pathlength Correction: Yes
  • Wavelength Accuracy: ±2 nm
  • Wavelength Repeatability: ±0.2 nm
  • OD Accuracy: <1% at 2.0 OD, <3% at 3.0 OD
  • OD Linearity: <1% from 0 to 3.0 OD
  • OD Repeatability: <0.5% at 2.0 OD
  • Stray Light: 0.03% at 230 nm
  • Reading Speed (Kinetic):
    • 96 wells: 14 seconds
    • 384 wells: 26 seconds

Fluorescence Intensity Mode

  • Light Source: Tungsten halogen (Xenon flash optional)
  • Detector: PMT
  • Wavelength Selection: Filters
  • Wavelength Range: 300 – 700 nm (option for 200 – 850 nm)
  • Dynamic Range: >6 decades
  • Sensitivity:
    • Top and Bottom: Fluorescein 5 pM (1 fmol/well, 96-well plate)
  • Reading Speed (Kinetic):
    • 96 wells: 31 seconds
    • 384 wells: 80 seconds

Luminescence Mode

  • Wavelength Range: 300 – 700 nm
  • Dynamic Range: >6 decades
  • Sensitivity:
    • 10 amol ATP (Flash) – Lum and Abs/Lum configurations
    • 30 amol ATP (Flash) – Multi-mode configurations

Time-Resolved Fluorescence Mode

  • Light Source: Xenon flash
  • Detector: PMT
  • Wavelength Selection: Monochromator

Alpha Detection Mode

  • Light Source: Tungsten halogen
  • Detector: PMT
  • Wavelength Selection: Filters
  • Sensitivity: 300 amol of biotinylated-LCK-P peptide (25 µL/well in 384-well plate)
  • Read Speed: 2 minutes (96-well plate)

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